microscopy slides 76  (Karl Hecht)

Journal: Communications Biology

Article Title: Unveiling the impact of hypodermis on gene expression for advancing bioprinted full-thickness 3D skin models

doi: 10.1038/s42003-024-07106-4

Figure Lengend Snippet: a Shear rate sweep and ( b ) temperature sweep of non-crosslinked collagen with and without fibroblasts. c Frequency sweep of crosslinked collagen with and without fibroblasts. d Time sweep at 37 °C of collagen solutions with and without fibroblasts. e Stress-strain curves of crosslinked collagen without fibroblasts, dermis (collagen + fibroblasts), and human skin equivalent (HSE). Scanning electron microscopy (SEM) of the crosslinked collagen ( f ) without and ( g ) with fibroblasts. Scanning electron micrographs of HSEH after 10 days of maturation. h shows the full thickness images, ( i ) shows the hypodermis region and ( j ) epidermis layer. In the graphs ( a – d ) error bars are resultant from n = 3.

Article Snippet: Transverse cuts were made with thickness of 5 µm and transferred to 76 × 26 mm microscopy slides (Waldemar Knittel).

Techniques: Shear, Electron Microscopy

Journal: Scientific Reports

Article Title: An automated real-time microfluidic platform to probe single NK cell heterogeneity and cytotoxicity on-chip

doi: 10.1038/s41598-021-96609-9

Figure Lengend Snippet: Experimental setup of high-throughput droplet-based cytotoxicity platform. ( A ) Experimental schematics showing cytotoxicity platform that combines (i) droplet generation and cell pairing using microfluidics, (ii) droplet immobilization for real-time microscopy, (iii) automated image analysis using custom-made MATLAB script to allow unbiased and high throughput detection of cytotoxic events. Stained NK cells and K562 cells were loaded into the chip using 200 µL pipette tips and encapsulated into droplets using a 3-inlet microfluidic chip. The viability dyes were included within the cell medium. The immobilized droplets were incubated in a stage top incubator set at 5% CO 2 and 37 °C. Image acquisition was performed at every hour interval for 10 h. ( B ) The three-inlet microfluidic device with flow-focusing junction to generate droplets. ( C ) A qualitative test of the observation chamber was performed by monitoring droplets movement in the chamber under the microscope for 10 h.

Article Snippet: Glass microscopy slides (76 × 26 × 1 mm; Corning) were used as top and bottom covers (76 × 26 × 1 mm).

Techniques: High Throughput Screening Assay, Microscopy, Staining, Transferring, Incubation

Journal: PLoS Neglected Tropical Diseases

Article Title: The physicochemical fingerprint of Necator americanus

doi: 10.1371/journal.pntd.0005971

Figure Lengend Snippet: (a) Time-lapse images of L 3 N . americanus exsheathing on poly-L-lysine coated glass slides (37°C). After an initial puncture, complete exsheathment occurs within 9 seconds (time is shown as minutes: seconds: milliseconds). Based on our observations N . americanus L 3 exsheathment occurs via a five-stage process: (I) Stimulation (00:00:00)–the N . americanus demonstrate heightened activity at 37°C, mimicking an encounter with human skin. (II) Thrashing (00:00:00–00:01:00)—larva move rapidly and violently. This behaviour expands the sheath in all directions to weaken its integrity and provides greater space for manoeuvre. (III) Puncture (00:02:00)—the anterior of the sheath wall is weakened, which can be attributed to the thrashing phase and possibly due to secreted enzymes that have been thought to digest the sheath membrane in its uppermost regions. The larva perforates the anterior sheath tip from which it can emerge. (IV) Exsheathment (00:02:00–00:08:00)—The larva manoeuvres out of the punctured sheath, through its characteristic sinusoidal movement, whilst the sheath remains anchored to the surface. The interface of the L 3 cuticle and sheath is indicated optically by a refractile ring. (V) Escape (00:09:00)–The nematode has completely exsheathed and is free to move independently of the sheath. For full movie see and for multiple exsheathments in a single field of view see and . (bi) Optical and (bii & biii) environmental scanning electron microscopy images for partially exsheathed N . americanus . Scale Bar (a) = 100 μm, (bi) = 100 μm (bi) 100 μm, (bii) = 50 μm (biii) = 10 μm.

Article Snippet: Methanol (HPLC grade), acetone (HPLC grade) and microscopy glass slides (76 x 26 x 1.0–1.2 mm), were purchased from Thermo Fisher Scientific (Hemel Hempstead, United Kingdom).

Techniques: Activity Assay, Membrane, Electron Microscopy